Document Type : Regular Articles
Authors
1
egypt Sohag University / Sohag Center
2
Chemistry Department, Faculty of Science, Sohag University, Egypt
3
3Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Member as researcher in Yousef Abdul Latiff Jameel.
4
Medicinal and Aromatic Plants Research Department, Horticulture Institute, Agricultural Research Center, Dokki, Giza, 12619, Egypt.
10.21608/sjsci.2025.423051.1309
Abstract
Moringa oleifera leaves (MOL), widely recognized for their nutritional and medicinal properties, are rich in natural antioxidants, proteins, vitamins, and essential minerals. The present study was conducted to evaluate the hepatoprotective and therapeutic potential of MOL extract against sodium nitrite (NaNO₂)-induced hepatotoxicity in adult male rats. Thirty-five rats were randomly allocated into five groups (n = 7): the control group, NaNO₂ group (75 mg/kg body weight), MOL group (400 mg/kg body weight), the therapeutic group (NaNO₂ for 21 days, followed by MOL), and the protective group (MOL for 21 days, followed by NaNO₂). Moreover, Gas chromatography-mass spectroscopy (GC-MS), Serum biomarkers of liver functions, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT), were measured. GC-MS exhibited the existence of varied bioactive compounds, while the main chemical constituents were 9-Octadecenami de (16.44%), Phenol, 2,2'-methylenebis [6-(1,1-dimethylethyl)-4-m ethyl (8.46%), 1,2,4-Metheno-1H-cycl obuta[cd]pentalene-3, 5-diol, octahydro (2.94%), 4H-1-benzopyran -4-one, 2-(3,4-dimethoxyp henyl)-3,5-dihydr oxy-7-methoxy (2.44%), NaNO₂ administration significantly elevated ALT, AST, ALP, and GGT (P < 0.001) compared with the control group, indicating severe liver damage. Conversely, MOL supplementation either before or after NaNO₂ exposure significantly ameliorated these alterations and improved hepatic histological architecture. These findings demonstrate that MOL exerts both protective and therapeutic effects against NaNO₂-induced liver injury, supporting its potential as a natural hepatoprotective agent.
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