Antimicrobial Activities of Biogenic Silver Nanoparticles Synthesized by Curvularia spicifera

Document Type : Regular Articles

Authors

Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag, 82524, Egypt.

Abstract

Curvularia spicifera (SUMCC 22003) is an endophytic fungus isolated from leaves of the medicinal plant Calotropis procera that were collected from Wadi Bir-EL-Ain, Sohag, Egypt. The fungus was identified based on morphology and phylogenetic analyses. The ability of C. spicifera to biosynthesize silver nanoparticles (AgNPs) was studied. The biosynthesized AgNPs were characterized using UV-vis spectroscopy, XRD measurement, DLS, Zeta potential analysis, FTIR and HR-TEM analysis. The formed AgNPs were stable, well-dispersed and spherical crystalline with an average diameter of 38.41 nm and a Zeta potential of -6.35 mV. The FTIR analysis confirmed that AgNPs are capped with protein. The biosynthesis optimization study indicated that 1 mM AgNO3, 5 g of biomass weight, pH 10.5 and a reaction temperature of 60°C were the optimal conditions for AgNPs biosynthesis. AgNPs exerted significant antimicrobial activity at different concentrations against the tested species of Gram-negative bacteria, Gram-positive bacteria and yeast species, demonstrating their potential as broad-spectrum antimicrobial agents. Escherichia coli showed the highest susceptibility to AgNPs (50 µg) with an inhibition zone diameter of 23.7±0.3 mm and MIC 4.2±0.1 µg. AgNPs (50 µg) exhibited an inhibition zone of 16.7±0.1 mm and MIC of 5.7±0.3 for Candida albicans.

Keywords

Main Subjects

Files

Share

How to cite

Statistics